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Our non-canonical nucleosome study has been updated. tl;dr - lots of VPP #cryoET; canonical nucleosomes account for < 10% of the total expected in situ. Work of Zhi Yang Tan, Shujun Cai, Alex Noble, and Jon Chen.
https://www.biorxiv.org/content/10.1101/2021.04.04.438362v2
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Happy to share Claris Chong's review on non-canonical nucleosome structures, now published at Chromosoma!
https://link.springer.com/article/10.1007/s00412-023-00791-w
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It's great to see that a popular #cryoEM software package is now a benchmark on a major enterprise hardware review website:
https://www.phoronix.com/review/intel-xeon-platinum-8490h/6
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Happy to share our in situ cryoET analysis of human chromosomes, in both G1 and metaphase. It's the work of Jon Chen , Tingsheng Liu, Shujun Cai, Cai Tong Ng, Jian Shi, and our collaborators Weimei Ruan and Uttam Surana.
https://www.biorxiv.org/content/10.1101/2023.07.31.551204v1
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🔥 Top post: Our non-canonical nucleosome study has been updated. tl;dr - lot · 9 likes + reposts
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Our non-canonical nucleosome study has been updated. tl;dr - lots of VPP #cryoET; canonical nucleosomes account for < 10% of the total expected in situ. Work of Zhi Yang Tan, Shujun Cai, Alex Noble, and Jon Chen.
https://www.biorxiv.org/content/10.1101/2021.04.04.438362v2
Come see our deeper dive into human chromatin in situ at #cellbio2023. #teamtomo study led by Jon Chen and Tingsheng Liu. Poster P2005, Board B281, Monday Dec 4. Preprint: https://www.biorxiv.org/content/10.1101/2023.07.31.551204v1.full
The nanoscale similarities and differences between G1 and metaphase chromatin are summarized in tables. Raw cryoET VPP data will be shared at EMPIAR. Now we need to figure out how cells control nucleosome structure and packing 🤔
G1 nucleosomes concentrate in domains, like what we saw in HeLa (https://www.molbiolcell.org/doi/10.1091/mbc.E18-05-0331) and what others saw w/ super-res. Metaphase nucleosomes are not surprisingly condensed in chromatids. Megacomplexes a…
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We find diverse canonical nucleosome structures in both G1 and metaphase. These include mononucleosomes, ordered stacked dinucleosomes, and nucleosomes with gyre-proximal (albeit heterogeneous) densities. Nucleosomes have variable linker-D…
Happy to share our in situ cryoET analysis of human chromosomes, in both G1 and metaphase. It's the work of Jon Chen , Tingsheng Liu, Shujun Cai, Cai Tong Ng, Jian Shi, and our collaborators Weimei Ruan and Uttam Surana.
https://www.biorxi…
Happy to share Claris Chong's review on non-canonical nucleosome structures, now published at Chromosoma!
https://link.springer.com/article/10.1007/s00412-023-00791-w
It's great to see that a popular #cryoEM software package is now a benchmark on a major enterprise hardware review website:
https://www.phoronix.com/review/intel-xeon-platinum-8490h/6
Hello, our fellow #chromatin, #mitosis, #cryoEM, and #cryoET friends! We're glad to have a presence here in addition to our science social-media home https://twitter.com/MitosisLab
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